Detection of pathogenic Escherichia coli on potentially contaminated beef carcasses using cassette PCR and conventional PCR

Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR

The aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains ofEscherichia coli in chicken carcasses by polymerase chain reaction (PCR). In this study 63 strains of E. coliwere isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughterhouse of Shahrekord. Polymerase chain reaction assays were used to detect t...

Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection†

Detection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR

Background and Aims: Many bacteria including Escherichia coli may enter into a viable but non-culturable (VBNC) state under unfavorable stresses, which are unable to be detected by culture-based methods. In this study, the use of Reverse Transcription PCR (RT-PCR) for detection of VBNC state of E. coli O157:H7 was investigated. Materials and Methods:  Escherichia. coli O157:H7 was inoculated i...

Escherichia coli detection using the ceramic PCR microsystem – first report

Escherichia coli (E.coli) is a common bacteria that colonizes digestive truck of many organisms including humans and is important source of Vitamin K and B12. However, numerous strains of E.coli are pathogenic and cause severe infections and alimentary toxemia. The main source of E.coli infections is water. That is why monitoring the purity of water sources is extremely important for public hea...

PCR for specific detection of H7 flagellar variant of fliC among extraintestinal pathogenic Escherichia coli.

A newly developed PCR-based assay for the H7 variant of the Escherichia coli flagellin gene, fliC, was 100% sensitive and specific in comparison with serology and probe hybridization. It revealed broad conservation of the H7 fliC variant among phylogenetically diverse lineages of extraintestinal pathogenic E. coli (ExPEC) and superseded serotyping for certain isolates with ambiguous or non-H7 s...